What Is The Coronavirus?

COVID-19 is an infectious disease caused by SARS-CoV-2. The outbreak of disease started in Dec. 2019 with the first case reported in China. The World Health Organization (WHO) recognised the outbreak of COVID-19 as a pandemic on 11 March 2020.

By the end of July 2020, there are over 17 million confirmed cases and close to 700 thousand deaths which have brought the economies of many countries to a halt. Countries that have implemented a global travel ban in response to the COVID-19 pandemic by April 2020 (above), later they gradually opened their borders. But there is still risk of the next waves of COVID-19 pandemic.

https://covid19.who.int/

Coronaviruses are crown-like respiratory viruses, replicating in the airways. Symptoms of infection can be mild, like the common cold, or more severe.

The median incubation time is estimated to be 5.1 days with symptoms expected to be present within 12 days of infection.

Diagnostic testing is possibly the only efficient way to know the spread of the SARS-CoV-2 in time and space, enabling policymakers and healthcare workers to track and mitigate the outbreak of COVID-1.

The WHO has appealed for global mass testing and defined molecular diagnostics as the gold standard for COVID-19 test.

Transmission electron micrograph of
SARS-CoV-2 virus particles.

How To Collect Samples ?

According to CDC Guidelines; specimen should be collected as soon as possible once a decision has been made to pursue testing, regardless of the time of symptom onset.

For initial diagnostic testing for SARS-CoV-2, CDC recommends collecting and testing an upper respiratory specimen. The following are acceptable specimens:

A nasopharyngeal (NP) specimen
An oropharyngeal (OP) specimen
A nasal mid turbinate swab (using a flocked tapered swab)
A n anterior nares (nasal swab)swab(using a flocked or spun polyester swab)
Nasopharyngeal wash/aspirate or nasal wash/aspirate (NW)

Swabs should be placed immediately into a sterile transport tube containing 2-3mL of either viral transport medium (VTM), Amies transport medium, or sterile saline, unless using a test designed to analyze a specimen directly, (i.e., without placement in VTM).

https://www.cdc.gov/coronavirus/2019 ncov/lab/guidelines clinical specimens.html

Molecular Diagnostics for Infection PCR

PCR (polymerase chain reaction) is a standard method for molecular diagnostics since 1990.
It requires specific expensive items of equipment and highly trained analysts and upwards of 4–8 h to process.

Molecular Diagnostics for Infection – Isothermal Amplification

Isothermal amplification method is led to save time in diagnosis. Five to six different types of isothermal amplification methods have been created. Among them LAMP (loop mediated isothermal amplification) is one of the widely experienced one.

Covidigen Multiplex qRT-PCR Test

We transform our 15 years experience into products to help the World fight against COVID-19 pandemic

Together with our experienced researchers, we combine our products that can be used in diagnosis of COVID-19 with the Covidigen qRT-PCR brand.

Our Products

Viral Transport Kit

Covidigen Viral Transport Kit is used for collection, and transport, of clinical specimens and long-term storage of viruses, such as Influenza, Chlamydia, Mycoplasma and Ureaplasma specimens.

The system is used for cell culture, Rapid Antigen Detection, PCR, and routine nucleic acid extraction. It is designed to maintain the optimum viability and virulence of the viral sample. It is mainly composed of a storage tube, preservation medium, and swabs.

Covidigen Viral Transport Kit is made of Hanks Balanced Salt Solution (HBSS) and contains protective protein antibiotics to control the overgrowth of bacteria and yeasts, maintain cellular integrity and encourage the preservation of viruses, chlamydia, and buffers to control the pH.

Product Name	Package Size	Cat. No
Covidigen Viral Transport Kit	100 tests	PH2020VTM-100

Nucleic Acid Purification Kit

COVID-19 RNA Purification Kit is intended for rapid purification of COVID-19 RNA from human oropharyngeal swab and nasopharyngeal swab specimens for in vitro diagnostic purposes. The purified viral RNA is suitable for RT-PCR, qRT-PCR, qPCR, viral detection, viral load determination and viral genotyping. Any diagnostic results produced in connection with any downstream diagnostic assay using this kit should be interpreted according to all relevant clinical and laboratory findings.

Product Name	Package Size	Cat. No
Covidigen Nucleic Acid Purification Kit	50 tests	NN202002-50
	100 tests	NN202002-100
	250 tests	NN202002-250

COVID-19 RNA Purification Kit utilizes a special lysis buffer in combination with silica based spin columns to efficiently purify viral RNA from the biological specimens.

Viral samples are lysed in optimized lysis buffer containing chaotropic salts. Binding buffer provides the selective binding condition of RNA to the silica matrix. The impurities such as proteins, salts and cellular debris are removed during subsequent washing and centrifugation steps.

Finally, highly pure nucleic acids are released with low ionic strength buffer. The purified RNA can be used directly in the most demanding downstream applications for in vitro diagnostic analysis. The entire procedure can be completed in 30 minutes.

COVID-19 Multiplex qRT PCR Kit

Covidigen COVID-19 Multiplex qRT-PCR Kit is a real-time RT-PCR test intended for the qualitative detection of nucleic acid from the SARS-CoV-2 in human oropharyngeal swab and nasopharyngeal swab specimens from individuals with signs and symptoms of infection who are suspected of COVID-19.

Covidigen COVID-19 Multiplex qRT-PCR Kit is intended for use by qualified and trained clinical laboratory personnel specifically instructed and trained in the techniques of real-time PCR and in vitro diagnostic procedures.

Product Name	Package Size	Cat. No
Covidigen COVID-19 Multiplex qRT-PCR Kit	100 tests	NN202001-100
Covidigen COVID-19 Multiplex qRT PCR Kit	500 tests	NN202001-500

This kit is a two channel (FAM and HEX) kit with detection of ORF1ab and N genes (two primer/probe sets)
Targets	Targeting the conservative sequences of ORF1ab and N gene
Sensitivity	For COVID-19 Target ORF1ab gene: 44 copies/ml For COVID-19 Target N gene: 79 copies/ml
Specifity	No cross-reactivity with SARS, MERS, etc.
Duration	Real-time reverse transcription PCR assay is completed in 38 minutes
Control	COVID-19 real-time PCR assay utilizes an internal control, which controls for target isolation and amplification
CE-IVD	For in-vitro diagnostic use

Please do not hesitate to contact our experts and visit our web sites for detailed information. Our experts would be greatful to inform you about our test kits.
+90 216 346 86 66

Pharmaline Sağlık Hizmetleri Ticaret A.Ş.
Atatürk Caddesi, Atatürk Cadde Konutları, No:43 A
Blok Daire:6 Sahrayıcedid Mahallesi
Kadıköy Istanbul-Türkiye